![]() ![]() The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN ABC Kit designated “Rabbit IgG”.įor additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate. Peroxidase (ABC, Elite ABC, RTU or Kits)Ģ) Choose the biotinylated secondary antibody:.The VECTASTAIN ABC Kits are available with a choice of two different enzyme systems Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:Īll applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN ABC system.Ĭhoosing a VECTASTAIN ABC Kit 1) Choose an enzyme system: This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. ![]() The VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. ![]()
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